SIMULTANEOUS MANIPULATION OF INTESTINAL CAPACITIES AND NUTRIENT LOADSIN MICE

To study the relationship between capacity and load in the small intes tine, we simultaneously varied dietary nutrient load and intestinal ca pacity in mice. Intestinal transection alone caused an increase in int estinal mass, because of increased serosal mass. Because virgin mouse intestine possesses 180% reserve uptake capacity before resection and the intestine regenerates after resection, resection of up to 50% had no effect on food intake, digestive efficiency, intestinal brush-borde r glucose uptake rate, or mass of all organs measured except the cecum . Regeneration of intestinal mass and glucose uptake capacity was quan titatively complete, because intestinal mass 10 wk after resection was similar to that in unresected mice. Resected intestinal mass in lacta ting mice was four times larger than that immediately after resection in virgin mice. Cecal mass increased in 50%-resected lactating mice wi th high food intakes, suggesting nutrient spillage into the distal gut as a signal for regeneration. Mice failed to survive 70% resection of the intestine, possibly because intestinal reserve uptake capacity wa s exhausted immediately after surgery, making regeneration impossible.