INTEGRATED DERIVATIZATION-CHEMILUMINESCENCE DETECTION SYSTEM FOR THE DETERMINATION OF BETA-CARBOLINE ALKALOIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

Citation
J. Cepas et al., INTEGRATED DERIVATIZATION-CHEMILUMINESCENCE DETECTION SYSTEM FOR THE DETERMINATION OF BETA-CARBOLINE ALKALOIDS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY, Journal of chromatography, 749(1-2), 1996, pp. 73-80
Citations number
21
Categorie Soggetti
Chemistry Analytical","Biochemical Research Methods
Journal title
Volume
749
Issue
1-2
Year of publication
1996
Pages
73 - 80
Database
ISI
SICI code
Abstract
An integrated derivatization-chemiluminescence detection system was us ed for the high-performance liquid chromatographic determination of be ta-carboline alkaloids such as harmol, harmalol, norharmane, harmane, harmine and harmaline by the chemiluminescence produced in their react ion with the bis(2,4-dinitrophenyl)oxalate (DNPO)-hydrogen peroxide sy stem. The ability of the proposed detection system based on its zero-d ead-volume to overcome the problems associated with the use of this ox alate ester in peroxyoxalate chemiluminescence detection in HPLC was e xploited to develop a sensitive chromatographic method for the determi nation of the above-mentioned hallucinogens. The method excels over ot her alternatives based on different detection techniques, such as UV a nd fluorescence spectroscopy, in terms of limits of detection. The pro posed method was validated by determining the alkaloids (the identific ation and quantification of some are reported for the first time) in H eliconiini butterfly specimens with good results.