LIQUID-CHROMATOGRAPHIC METHOD FOR THE DETERMINATION OF NICOTINE IN PHARMACEUTICAL FORMULATIONS

A simple high-performance liquid chromatography method was developed a nd validated for the analysis of nicotine in various pharmaceutical fo rmulations. This method required a simple liquid-liquid extraction pro cedure prior to liquid chromatography analysis. The chromatographic se paration was achieved on a reversed-phase C-18 column with ultraviolet detection at 260 nm. This isocratic system was operated at ambient te mperature and required 10 min of chromatographic time. The mobile phas e consisted of methanol-citrate phosphate buffer (15:85, v/v) at a flo w-rate of 0.7 ml/min. Standard curves were linear over the concentrati on range 1.0-51 mu g/ml. Within-day and day-to-day relative standard d eviations ranged from 1.3 to 4.4% and from 2.4 to 4.2%, respectively.