UTILIZATION OF N-ALKYL-BETA-D-GLUCOPYRANOSIDES IN ENANTIOMERIC SEPARATION BY MICELLAR ELECTROKINETIC CHROMATOGRAPHY

The n-octyl-beta-D-glucopyranoside has been previously used in micella r liquid chromatography to separate racemic mixtures. We report here o n the utilization of n-alkyl-beta-D-glucopyranosides (from C-7 to C-10 ) as pseudo stationary phases in the separation by HPCE of racemic mix tures of amino acids, derivatized as carbamates. The result from this comparative study was that the best enantiomeric selectivity is obtain ed with the n-nonyl-beta-D-glucopyranoside. Using this chiral surfacta nt we optimized different operating parameters such as: concentration of surfactant, ionic strength and pH of the mobile phase and temperatu re. Therefore it appeared that this new electrophoretic system is perf orming best at 18 degrees C, with an 80 mM concentration of surfactant in the electrolyte constituted by a NaHCO3-NaOH buffer, the applied v oltage being equal to 15 kV. The enantiomeric selectivity of this syst em, original because of the nature of the micellar additive (neutral m icelle), seems to be a function of the analyte hydrophobicity.