H. Berghammer et al., NUCLEOTIDE-SEQUENCE OF UB52 FROM THE CNIDARIAN ACROPORA-MILLEPORA REVEALS HIGH EVOLUTIONARY CONSERVATION, Gene, 178(1-2), 1996, pp. 195-197
We report the cDNA sequence encoding Ub52 from the cnidarian Acropora
millepora. As in other eukaryotes, the N-terminal region of the deduce
d amino-acid sequence is a ubiquitin moiety and the C-terminal region
CEP52, a protein component of the large ribosomal subunit. A. millepor
a Ub52 is highly homologous (>95% identical) with the corresponding Dr
osophila and vertebrate sequences, the cnidarian sequence having highe
r identity with these 'higher' metazoan homologs than does the corresp
onding Caenorhabditis elegans protein. However, the cnidarian sequence
contains two unique substitutions in the ubiquitin moiety, Ser(22) an
d Thr(28). Northern analysis indicates that A. millepora Ub52 is encod
ed by a small (<800 nt) mRNA present in eggs and is first expressed af
ter the early gastrula stage in embryonic development.