Three laccase-encoding cDNAs were cloned from a tobacco stem cDNA libr
ary. One of them contains a full length sequence coding for a cationic
laccase. The predicted polypeptide sequence shows 48% identity with s
ycamore laccase. Amino acid comparisons with other laccases and ascorb
ate oxidases have shown that this new plant laccase sequence also cont
ains four potential copper binding regions which are highly conserved
among the blue copper oxidases.