GENE OF RAT CA2+ CALMODULIN-DEPENDENT PROTEIN-KINASE-II ALPHA-ISOFORM- ITS CLONING AND WHOLE STRUCTURE/

The gene encoding the ex isoform of rat Ca2+/calmodulin-dependent prot ein kinase II was cloned, and its exon-intron organization was analyze d. The coding region of cDNA consists of 18 exons spanning more than 5 0 kilobase pairs, Each of the discrete functional units, such as the A TP-binding site, the autophosphorylation site responsible for Ca2+-ind ependent activity, the calmodulin-binding site, and link structure is encoded by a single exon, The largest and smallest exons consist of 22 9 and 41 base pairs, respectively, All splice junction sequences flank ing the introns conform to the consensus splice junction sequence and the GT-AG splice rule.