GENETIC DIVERSITY OF HUMAN PARVOVIRUS B19 - SEQUENCE-ANALYSIS OF THE VP1 VP2 GENE FROM MULTIPLE ISOLATES/

To evaluate the genetic variability of human parvovirus B19, the compl ete coding region of the VP1/VP2 structural proteins of 29 B19 isolate s obtained from 25 infected patients were sequenced and compared with each other and with two previously published gig isolates. The VP1/VP2 gene was amplified by PCR using B19-specific oligonucleotide primers and the amplification products were sequenced directly. Overall, the a verage nucleotide and predicted amino acid identity among B19 isolates was high. Sequential virus isolates from the same cases and isolates obtained from two cases linked by transmission in the same household w ere essentially identical. Sequence variation was minimal among isolat es obtained from a single community-wide gig outbreak, ranging between 0 and 10 (0 . 4%) base substitutions, although there appeared to be m ore than one genetic lineage circulating in the outbreak. A comparison with 18 additional isolates from distinct epidemiological settings fo und greater variability. These isolates differed from each other by be tween 11 (0 . 5%) and 112 (4 . 8%) base substitutions. B19 isolates fr om Xi'an, China, were significantly different from other isolates at b oth the nucleotide and amino acid levels, and were more closely relate d to a single isolate from Japan, obtained 10 years earlier, than to i solates from other countries. Isolates examined in this study included distinct genotypes from patients with similar clinical presentations and similar genotypes from patients with diverse clinical presentation s. These data suggest that geographically defined genetic lineages of B19 may exist and that no particular B19 genotype was associated with a particular clinical outcome.