FROM NOESY CROSS PEAKS TO STRUCTURAL CONSTRAINTS IN A PARAMAGNETIC METALLOPROTEIN

Authors
BERTINI I DONAIRE A FELLI IC ROSATO A LUCHINAT C
Citation
I. Bertini et al., FROM NOESY CROSS PEAKS TO STRUCTURAL CONSTRAINTS IN A PARAMAGNETIC METALLOPROTEIN, Magnetic resonance in chemistry, 34(11), 1996, pp. 948-950
Citations number
20
Categorie Soggetti
Spectroscopy,Chemistry
Journal title
Magnetic resonance in chemistry → ACNP
ISSN journal
07491581
Volume
34
Issue
11
Year of publication
1996
Pages
948 - 950
Database
ISI
SICI code
0749-1581(1996)34:11<948:FNCPTS>2.0.ZU;2-3
Abstract
A method to take into account the effect of paramagnetic enhancements of nuclear relaxation rates when extracting distance constraints from the intensity of dipolar connectivities is presented, It is based on r ecalculating the intensity of NOESY cross peaks from a structure with consideration of paramagnetic relaxation. The method can be integrated into any standard structure calculation procedure, and provides a sig nificant improvement of resolution in the neighborhood of the paramagn etic center.