A review of the fundamental aspects and environmental applications of
biosensors is presented. The bases of different transducer principles
such as electrochemical, optical and piezoelectric are discussed. Vari
ous examples are given of the applications of such principles to devel
op immunosensor devices to determine common environmental contaminants
. Attention is also paid to catalytic biosensors, using enzymes as sen
sing elements. Biosensor devices based on the use of cholinesterase an
d various oxidase enzymes such as tyrosinase, laccase, peroxidase and
aldehyde dehydrogenase are reported. Some examples are given of the ap
plications of other biomolecules such as whole cells, DNA or proteins,
to determine pollution. Validation studies are presented comparing bi
osensors with chromatographic techniques to determine organophosphorus
pesticides and phenolic compounds in environmental samples.