UTILITY OF A TEST FOR CHROMOSOMAL MALSEGREGATION IN SACCHAROMYCES-CEREVISIAE STRAIN D61.M FOR THE DETECTION OF ANTIANEUGENS - TEST OF THE MODEL COMBINATION OF CHLOROPHYLLIN AND NOCODAZOLE
A. Verma et al., UTILITY OF A TEST FOR CHROMOSOMAL MALSEGREGATION IN SACCHAROMYCES-CEREVISIAE STRAIN D61.M FOR THE DETECTION OF ANTIANEUGENS - TEST OF THE MODEL COMBINATION OF CHLOROPHYLLIN AND NOCODAZOLE, Mutation research, 358(1), 1996, pp. 73-80
Despite the fact that aneuploidy is a major genetic cause of human mor
bidity and mortality, antimutagenicity studies have used predominantly
short-term tests that detect gene mutations, chromosomal aberrations,
and micronuclei. Therefore, the major deficiency in the use of short-
term tests for antimutagenicity studies is those that detect chromosom
al malsegregation leading to aneuploidy. Thus, we initiated a study on
the utility of short-term tests for the detection of antianeugenic ac
tivity. We selected strain D61.M of Saccharomyces cerevisiae, nocodazo
le, and chlorophyllin as a model short-term test, aneugen, and antimut
agen, respectively, for our initial study. Chlorophyllin strongly inhi
bited the aneugenic activity of nocodazole, but had no effect on the e
ndpoints when tested alone, in strain D61.M. To our knowledge, this is
the first report of an antianeugen. Furthermore, we conclude that str
ain D61.M can be used as a relatively simple, inexpensive, and rapid s
hort-term test for the study of antianeugenicity.