Ch. Li et al., ATPASE ACTIVITY OF THE CYSTIC-FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR, The Journal of biological chemistry, 271(45), 1996, pp. 28463-28468
The gene mutated in cystic fibrosis codes for the cystic fibrosis tran
smembrane conductance regulator (CFTR), a cyclic AMP-activated chlorid
e channel thought to be critical for salt and water transport by epith
elial cells. Plausible models exist to describe a role for ATP hydroly
sis in CFTR channel activity; however, biochemical evidence that CFTR
possesses intrinsic ATPase activity is lacking, In this study, we repo
rt the first measurements of the rate of ATP hydrolysis by purified, r
econstituted CFTR. The mutation CFTRG551D resides within a motif conse
rved in many nucleotidases and is known to cause severe human disease.
Following reconstitution the mutant protein exhibited both defective
ATP hydrolysis and channel gating, providing direct evidence that CFTR
utilizes ATP to gate its channel activity.