BACTERIAL-INFECTIONS OF HEMOCYTES ASSOCIATED WITH THE MATERNALLY INHERITED MALE-KILLING TRAIT IN BRITISH POPULATIONS OF THE 2 SPOT LADYBIRD, ADALIA-BIPUNCTATA
Gdd. Hurst et al., BACTERIAL-INFECTIONS OF HEMOCYTES ASSOCIATED WITH THE MATERNALLY INHERITED MALE-KILLING TRAIT IN BRITISH POPULATIONS OF THE 2 SPOT LADYBIRD, ADALIA-BIPUNCTATA, Journal of invertebrate pathology, 68(3), 1996, pp. 286-292
Adalia bipunctata, the two spot ladybird, carries a vertically transmi
tted bacterial agent which kills male progeny during embryogenesis. So
me matrilines of A. bipunctata give rise to strongly female-biased sex
ratios, 16S rDNA sequence analysis revealed a bacterium of the genus
Rickettsia associated with this trait, a conclusion which is corrobora
ted here, Using light microscopy, an association between a bacterium l
ocated in A. bipunctata hemocyte cytoplasm and matrilines which show t
he sex ratio trait was found. This element was not found in hemocytes
taken from females from normal sex ratio lines, nor in hemocytes taken
from males. The association is confirmed by study of the inheritance
of the sex ratio trait. Only daughters of sex ratio crosses that bear
this cytoplasmic bacterium also show the sex ratio trait, with other d
aughters being normal with respect to sex ratio. Transmission electron
microscopy of hemocytes revealed a walled bacterium, bearing features
of members of the genus Rickettsia, free in the cytoplasm of hemocyte
s taken from infected lines, but not in those taken from uninfected li
nes. (C) 1996 Academic Press, Inc.