HETEROLOGOUS EXPRESSION OF G-PROTEIN-COUPLED RECEPTORS

G-protein-coupled receptors (GPCRs) are integral membrane proteins of great pharmacological importance owing to their central role in the re gulation of cellular responses to external stimuli. Heterologous expre ssion systems have been used to explore ligand binding, G protein and effector coupling, and structural aspects of the receptors, GPCRs can be expressed in a functional form in all expression systems, but with varying degrees of success because of differences in receptor and host cell characteristics. This article will discuss aspects related to th e choice and suitability of expression systems for the intended analys is of GPCR properties.