Biomass yield of microorganisms is important in applied microbiology s
ince it is the ultimate factor determining the amount of product produ
ced regardless of whether product is growth-linked or not. In the case
of environmental microbiology the opposite is true and minimizing the
biomass produced, or the sludge in the relevant jargon, often is the
prime goal. In this paper, a unique means of manipulating the microbia
l biomass yield of a heterogeneous culture to fulfil either of the two
goals is presented. 5.0 mgl(-1) Ni(II) in the feed composition to a c
ompletely mixed, once- through, activated sludge was found to induce t
he observed biomass yield of the microbial culture developed from sewa
ge. As compared with the base-line study without Ni(LI), where the rea
ctor received synthetic wastewater only, true biomass yield was found
to have increased along with the increased decay constant with the net
effect of lowering observed biomass yield drastically at lower diluti
on rates and increasing it over that observed in the base-line study a
t higher dilution rates. At 10.0 mgl(-1) influent Ni(II) concentration
the culture conditions almost reverted back to the base- line study a
nd at 25 mgl(-1) Ni(II) concentration a truly steady-state condition c
ould not be attained. Copyright (C) 1996 IAWQ.