QUANTITATIVE MALDI-TOF MS OF OLIGONUCLEOTIDES AND A NUCLEASE ASSAY

Authors
SARRACINO D RICHERT C
Citation
D. Sarracino et C. Richert, QUANTITATIVE MALDI-TOF MS OF OLIGONUCLEOTIDES AND A NUCLEASE ASSAY, Bioorganic & medicinal chemistry letters, 6(21), 1996, pp. 2543-2548
Citations number
25
Categorie Soggetti
Chemistry Inorganic & Nuclear","Chemistry Medicinal
Journal title
Bioorganic & medicinal chemistry letters → ACNP
ISSN journal
0960894X
Volume
6
Issue
21
Year of publication
1996
Pages
2543 - 2548
Database
ISI
SICI code
0960-894X(1996)6:21<2543:QMMOOA>2.0.ZU;2-M
Abstract
DNA oligomers 8 and 12 nucleotides long have been detected quantitativ ely by MALDI-TOF mass spectrometry using longer oligonucleotides as in ternal standards. Employing this method, the kinetics of the nuclease degradation of the octamer were analyzed. Reactions performed in the p resence of the ssDNA-binding peptide KWK yielded a footprint based on fragments from both the 3'- and the 5'-terminus of the oligonucleotide . Copyright (C) 1996 Elsevier Science Ltd