The herbicide isoproturon was degraded rapidly in a sandy loam soil un
der laboratory conditions (incubation temperature, 15 degrees C; soil
moisture potential, -33 kPa). Degradation was inhibited following trea
tment of the soil with the antibiotic chloramphenicol, but unaffected
by treatment with cycloheximide, thus indicating an involvement of soi
l bacteria. Rapid degradation was not observed with other phenylurea h
erbicides, such as diuron, linuron, monuron or metoxuron incubated in
the same soil under the same experimental conditions. Three successive
applications of isoproturon to ten soils differing in their physicoch
emical properties and previous cropping history induced rapid degradat
ion of the herbicide in most of them under laboratory conditions. Ther
e were, however, no apparent differences in ease of induction of rapid
degradation between soils which had been treated with isoproturon for
the last five years in the field and those with no pre-treatment hist
ory. A mixed bacterial culture able to degrade isoproturon in liquid c
ulture was isolated from a soil in which the herbicide degraded rapidl
y.