ESTIMATION OF THE OPTIMAL CONCENTRATIONS OF RESIDUAL SUGAR AND CELL-GROWTH RATE FOR A FED-BATCH CULTURE OF SACCHAROMYCES-CEREVISIAE

Estimation of the optimal concentrations of residual sugar in medium f or a fed-batch culture of Baker's yeast has been studied and practiced . The concentrations, however, depended on different species and targe ts of the biomass, which was expected to be made. Kinetic changes of t he residual phosphate salt in the medium conformed to a logarithmic pr ocess until the fourth hour during an 11-h culture. The parabolic meth od (see ref. 9 later in article) might be qualified to maintain the co ncentrations of residual sugar around 0.15 g/L. It was demonstrated th at cell growth followed a sigmoid process during a fed-batch culture, because the cells consumed the nutrient with two metabolic pathways, o ne was for cell conversion and another was for non-cell conversion. Wi th the parabolic method, we can estimate kinetics of cell growth and c ell growth rate during the culture.