A simple method based on Schiff's base formation between p-dimethylami
nobenzaldehyde and cephalosporin C is developed for estimation of ceph
alosporin C. The calibration curve was linear up to 500 mu g of cephal
osporin C. The application of the method in monitoring bioconversion o
f cephalosporin C to glutaryl-7-amino cephalosporanic acid is describe
d.