THE EVIDENCE FOR THE INCORPORATION OF FULVIC-ACID INTO THE BONE AND CARTILAGE OF RATS

In animal testing, abdominal injection of commercial fulvic acid (FA) to Wistar rats resulted in the coloration of the bone. Free radical si gnal was detected by ESR spectra of the bone, which were shown to be d ependent upon the dosage of FA injected. The fluorescence spectra, UV spectra and gel permeation chromatography of the alkaline extracts of the bone showed similar characteristics to FA. In in-vivo rat tests an d in-vitro experiments of cultured chicken cartilage tissue with triti um labeled FA, the results showed that H-3-FA could be incorporated in to the bone and cartilage of rats.