A NEW SITE-SPECIFIC ENDONUCLEASE FROM ACINETOBACTER SPECIES (STRAIN-M)

Authors
ZELINSKAYA NV KOVALEVSKAYA NP MATVIENKO NN ZHELEZNAYA LA MATVIENKO NI
Citation
Nv. Zelinskaya et al., A NEW SITE-SPECIFIC ENDONUCLEASE FROM ACINETOBACTER SPECIES (STRAIN-M), Biochemistry, 61(8), 1996, pp. 1042-1051
Citations number
11
Categorie Soggetti
Biology
Journal title
Biochemistry → ACNP
ISSN journal
00062979
Volume
61
Issue
8
Year of publication
1996
Pages
1042 - 1051
Database
ISI
SICI code
0006-2979(1996)61:8<1042:ANSEFA>2.0.ZU;2-1
Abstract
The site-specific endonuclease R . AspMI was isolated from Acinerobact er species (strain M) and purified to functional purity. The enzyme re cognizes the symmetrical DNA sequence 5'-AGG down arrow CCT-3' and cle aves it as shown by the arrow, forming blunt DNA ends. The enzyme is a n isoschizomer of endonuclease StuI. Cleavage of the DNA site is inhib ited by dcm-methylation. AspMI is in solution as an similar to 30-kD m onomer.