Pl. Kinney et al., BIOMARKERS OF LUNG INFLAMMATION IN RECREATIONAL JOGGERS EXPOSED TO OZONE, American journal of respiratory and critical care medicine, 154(5), 1996, pp. 1430-1435
Citations number
19
Categorie Soggetti
Emergency Medicine & Critical Care","Respiratory System
Humans exhibit an acute inflammatory response in the lungs after contr
olled laboratory exposure to ozone. The present study was designed to
test whether biomarkers of inflammation are detectable in humans expos
ed to ozone and associated copollutants under natural conditions outdo
ors. Bronchoscopy with bronchoalveolar lavage (BAL) was carried out on
19 normal volunteer joggers from Governors Island, NY, who exercised
in the afternoon during the 1992 summer (S1) season. Fifteen subjects
were retested during the following, low ozone, winter season (W). The
BAL protocol involved an initial instillation of 20 ml saline followed
by four sequential 50-ml saline washes carried out in both the right
middle lobe and the lingula. The eight 50-ml samples were pooled as th
e ''alveolar'' sample. Analyses performed on the alveolar lavage sampl
es included cell differentials, release of IL-8, TNF-alpha, and reacti
ve oxygen species (ROS) by pooled cells, and levels of IL-8, protein,
LDH, fibronectin, alpha 1-antitrypsin (alpha 1-AT), complement fragmen
t 3a (C3a), and prostaglandin E(2) (PGE(2)) in lavage fluids. Release
of ROS by stimulated BAL cells was lower in S1 than in W (p = 0.03). I
n contrast, LDH levels in BAL fluids were 2-fold higher in 51 than in
W (p = 0.02), as were IL-8 (p = 0.12) and PGE(2) (p = 0.06). These res
ults suggest a possible ongoing inflammatory response in the lungs of
recreational joggers exposed to ozone and associated copollutants duri
ng the summer months.