PREPARATION OF DEXTRAN IMMUNOLOGICAL MAGNETIC NANOPARTICLES AND THEIRAPPLICATION TO COMBINED TARGETING CARRIER

Superparamagnetic dextran nanoparticles were prepared by coprecipitati on. Effects of concentration of dextran, amount of ironic salts, stirr ing speed, concentration of ammonium hydroxide and mole ratio of Fe3+/ Fe2+ on the effective diameter of dextran magnetic nanoparticles (DMNP ) were studied. Dextran immunological magnetic nanoparticles (DIMNP) w ere formed by the reaction of the monoclonal anti-human mammary cancer antibody with DMNP oxidized by sodium periodate, and the properties o f magnetic susceptibility, shape and retention of antibody activity we re investigated. The in vitro cancer antigen binding ability of DIMNP was demonstrated by radioimmunoassay, and the in vivo magnetic localiz ation and antibody targeting ability of radiolabeled DIMNP were studie d.