Sj. Bao et al., PRELIMINARY CRYSTALLOGRAPHIC ANALYSIS OF RECOMBINANT CHICKEN LIVER FRUCTOSE-2,6-BISPHOSPHATASE, Science in China. Series B, Chemistry, life sciences & earth sciences, 39(1), 1996, pp. 7-10
The bisphosphatase domain of chicken liver phofructo-2-kinase/fructose
-2,6-bisphosphosphatase was expressed in high yield Escherichia coli a
nd purified to homogeneity. Single crystals of chicken liver bisphopha
tase domain suitable for X-ray diffraction were obtained by the hangin
g drop The crystals belong to tetragonal space group P(4)2(1)2 or P4(3
)2(1)3 with two molecules per asymmetric unit. The determined cell dim
ensions are: a=b=10.02 nm, c=13.98 nm, alpha=beta=Gamma=90 degrees Dif
fraction data were collected on Weissenberg camera with synchrotron ra
diation at 0.32 nm resolution.