PREVALENCE OF MYCOPLASMA CONSERVED DNA IN MALIGNANT OVARIAN-CANCER DETECTED USING SENSITIVE PCR-ELISA

Mycoplasmas are tiny polymorphic prokaryotic organisms (0.2-0.3 mu m) that lack a cell wall and reside ubiquitously at the cell membrane or internalized into the cell. The organisms have been implicated in many diseases including functioning as cofactors catalyzing the HIV diseas e state. The oncogenic potential of mycoplasmas was only recently real ized when they were shown to cause chromosomal changes and in vitro ce ll transformations through gradual progressive chromosomal loss and tr anslocations. While a recent study linked mycoplasmas with gastric can cer, the association between mycoplasmas and ovarian cancer has not be en established, Recently, a commercial assay which combined polymerase chain reaction (PCR) and enzyme-linked immunosorbent assay (ELISA) me thods was developed for the detection of mycoplasmas. The present obje ctive was to determine the prevalence of mycoplasmas in archived paraf fin-embedded malignant ovarian cancer tissue. The combined PCR-ELISA p rocedure was used with consensus primers targeting for 15 species of m ycoplasmas and acholeplasmas. Archived human malignant ovarian cancer tissues (N = 27 cases) embedded in paraffin blocks were processed, and DNA was extracted and the presence of DNA verified. The extracted DNA specimens were randomly divided into three groups for analyses, PCR-E LISA assays were performed on extracted DNA together with appropriate negative and positive controls. The results showed mycoplasmas were pr esent in 59.3% of the malignant ovarian cancer specimens, PCR-ELISA an alysis of Neisseria gonorrhea and Chlamydia trachomatis controls did n ot produce cross-reacting false-positive results. The results suggest an association between mycoplasmas and malignant ovarian cancer. A 59. 3% prevalence rate was demonstrated for mycoplasmas in paraffin-embedd ed ovarian cancer tissues, The mechanism involved in oncogenesis by my coplasmas remains to be elucidated. (C) 1996 Academic Press, Inc.