CHARACTERIZATION OF RAINBOW-TROUT, ONCORHYNCHUS-MYKISS (WALBAUM), SERUM CREATINE-KINASE ISOENZYMES AND ISOFORMS BY MEANS OF ELECTROPHORESISAND ISOELECTRIC-FOCUSING

Serum samples were obtained from rainbow trout, Oncorhynchus mykiss (W albaum), from a commercial fish farm. Creatine kinase (CK; EC 2.7.3.2) isoenzymes and sub-bands (isoforms) were studied using agarose gel el ectrophoresis and polyacrylamide gel isoelectric focusing. Creatine ki nase isoenzymes and isoforms (sub-bands) were found to display dispara te electrophoretic mobility and isoelectric points, respectively when compared with CK patterns of human origin. The CK-BB isoenzyme, rarely detected in human serum, was detected in all trout samples assayed. U p to eight CKMM isoforms were detected by the polyacrylamide gel IEF m ethod described.