MOLECULAR REPLACEMENT STUDY ON FORM-B MONOCLINIC CRYSTAL OF INSULIN

Citation
Jh. Ding et al., MOLECULAR REPLACEMENT STUDY ON FORM-B MONOCLINIC CRYSTAL OF INSULIN, SCIENCE IN CHINA SERIES C-LIFE SCIENCES, 39(2), 1996, pp. 144-153
Citations number
14
Categorie Soggetti
Biology
ISSN journal
10069305
Volume
39
Issue
2
Year of publication
1996
Pages
144 - 153
Database
ISI
SICI code
1006-9305(1996)39:2<144:MRSOFM>2.0.ZU;2-5
Abstract
The form-B monoclinic insulin crystal was obtained from the sodium cit rate buffer with 1% zinc chloride, keeping phenolic content between 0. 76% and 1.25%. Its space group is P2(1), cell constants are: a=4.924nm , b-6.094nm, c=4.818nm, beta=95.8 degrees. There are 6 insulin molecul es which form a hexamer. The initial phase was obtained by Lsing rotat ion function program of X-PLOR program package and molecular packing p rogram of our laboratory. The molecular model was chosen from 4 zinc b ovine insulin hexamer. After the preliminary refinement by using the m acromolecular rigid body refinement technique, the molecular model was further refined and adjusted by using the energy-minimizing stereoche mically restrained least-squared refinement on the difference Fourier maps, The final R-factor is 22.4% at 0.3 nm resolution, the r.m.s. dev iations from standard bond length and bond angle are 0.0022 nm and 4.7 degrees, respectively.