NATURAL-KILLER-CELL CYTOTOXICITY ASSAY WITH TIME-RESOLVED FLUOROMETRY

A new time-resolved fluorimetric method for the measurement of natural killer (NK) cell cytotoxicity has been developed by labelling the tar get cell K562 with a new synthesized fluorescence marker KLUK. The met hod has advantages of higher sensitivity, time-saving, good reproducib ility and has no radioactivity problems. A satisfactory result is obta ined by comparing it with Cr-51 release method. It demonstrates that t he new marker provides an alternative to currently used radioactive ma rkers for the assessment of in vitro cellular cytotoxicity.