The retrograde intraaxonal transport of fluorescent dyes (10% Bisbenzi
mide or 2% Nuclear Yellow) was used to identify the motoneuron populat
ions that control two prime movers of the rat shoulder joint, the acro
miodeltoid (Ad) and spinodeltoid (Sd) muscles. The Ad and Sd motor nuc
lei were both comprised of an average of 30-40 motoneurons distributed
between spinal segments C5 and C7, in lateral regions of Rexed's lami
na IX. Although there was considerable overlap among them, the center
of the Ad motor nucleus was consistently located more rostral and medi
al than that for the Sd motor nucleus.