ANALYSIS OF CONNEXIN43 GENE-EXPRESSION INDUCED BY RETINOIC ACID IN F9TERATOCARCINOMA CELLS

Citation
A. Clairmont et al., ANALYSIS OF CONNEXIN43 GENE-EXPRESSION INDUCED BY RETINOIC ACID IN F9TERATOCARCINOMA CELLS, FEBS letters, 397(1), 1996, pp. 22-24
Citations number
30
Categorie Soggetti
Biophysics,Biology
Journal title
ISSN journal
00145793
Volume
397
Issue
1
Year of publication
1996
Pages
22 - 24
Database
ISI
SICI code
0014-5793(1996)397:1<22:AOCGIB>2.0.ZU;2-V
Abstract
All-trans retinoic acid (10(-7) M) induces cell-cell communication and expression of the gap junction protein connexin43 in mouse F9 teratoc arcinoma cells. Northern blot analysis revealed an increase of connexi n43 mRNA after treatment with retinoic acid, accompanied by an increas e of the mRNA of collagen IV, a differentiation marker. To address the question at what level gene expression is enhanced by retinoic acid, nuclear run-on experiments were carried out. There was no detectable c hange in the level of newly transcribed connexin43 mRNA. Therefore, we postulate a post-transcriptional mechanism responsible for the regula tion of connexin43 mRNA levels by retinoic acid.