HIGH ENDOGENOUS CALCIUM BUFFERING IN PURKINJE-CELLS FROM RAT CEREBELLAR SLICES

1. The ability of Purkinje cells to rapidly buffer depolarization-evok ed intracellular calcium changes (Delta[Ca2+](i)) was estimated by tit rating the endogenous buffer against incremental concentrations of the Ca2+-sensitive dye fura-2. 2. In cells from 15-day-old rats, pulse-ev oked Delta[Ca2+](i) were stable during the loading with 0.5 mM fura-2 through the patch pipette. In cells from 6-day-old rats, Delta[Ca2+](i ) decreased by similar to 50% during equivalent experiments. This decr ease was not related to changes in Ca2+ influx, since the integral of the Ca2+ currents remained constant throughout the recording. 3. Exper iments with high fura-2 concentrations (1.75-3.5 mM) were performed in order to obtain for each cell the curve relating Delta[Ca2+](i) to fu ra-2 concentration. From this relationship, values for the Ca2+ bindin g ratio (the ratio of buffer-bound Ca2+ changes over free Ca2+ changes ) were calculated. 4. In Purkinje cells from 15-day-old rats, the Ca2 binding ratio was similar to 2000, an order of magnitude larger than that of other neurones and neuroendocrine cells studied to date. This Ca2+ binding ratio was significantly smaller (similar to 900) in Purki nje cells from 6-day-old-rats. 5. We propose that the large Ca2+ bindi ng ratio of Purkinje cells is related to the presence of large concent rations of Ca2+ binding proteins and that these cells regulate their a bility to handle Ca-i(2+) loads during development through changes in the concentration of Ca2+ binding proteins.