INTERACTION OF RABPHILIN3 WITH SYNAPTIC VESICLES THROUGH MULTIPLE REGIONS

Rabphilin3 is a downstream target protein of Rab3A small G protein, im plicated in neurotransmitter release. We have previously reported that about 4 molecules of Rabphilin3 are associated with one synaptic Vesi cle through a vesicle anchoring protein(s). We have shown here that Ra bphilin3 interacts with rat synaptic vesicles through at least three r egions: the N-terminal region (1-280 amino acids (aa)), the C-terminal region containing the two C2 domains (440-704 aa), and an unidentifie d region. A model for the interaction of Rabphilin3 with the vesicles is discussed. (C) 1996 Academic Press, Inc.