CDK5 EXPRESSION AND ASSOCIATION WITH P35(NCK5A) IN EARLY STAGES OF RAT CEREBELLUM NEUROGENESIS - TYROSINE DEPHOSPHORYLATION AND ACTIVATION IN POSTMITOTIC NEURONS
Jb. Lazaro et al., CDK5 EXPRESSION AND ASSOCIATION WITH P35(NCK5A) IN EARLY STAGES OF RAT CEREBELLUM NEUROGENESIS - TYROSINE DEPHOSPHORYLATION AND ACTIVATION IN POSTMITOTIC NEURONS, Neuroscience letters, 218(1), 1996, pp. 21-24
We have examined the expression of cyclin dependent kinase (cdk) 5 pro
tein kinase and p35(nck5a). its activator subunit, during postnatal ne
urogenesis in rat cerebellum, using mono-specific antibodies. Both cdk
5 and p35(nck5a) are present and associated in proliferative stapes, a
lthough cdk5-p35 kinase activity is barely detectable. Cdk5-p35 activi
ty, but not the expression of either subunit, increases up to B-fold d
uring neuronal differentiation. Since we observe that cdk5 is phosphor
ylated on tyrosine in proliferative, bur not in post-mitotic stages, w
e suggest that post-translational regulatory mechanisms control cdk5-p
35 protein kinase activity during neurogenesis.