A 300 MHz high-resolution, high-pressure NMR probe which operates in t
he pressure range of 1 bar to 9 kbar at temperatures of -30 to 100 deg
rees C is described. Specialized novel design features of the probe ar
e discussed and test spectra showing resolution better than 1 Hz(<3.0
X 10(-9)) for 8 mm samples are presented. Potential biochemical applic
ations of this probe are illustrated by experiments dealing with the p
ressure-induced unfolding of hen egg white lysozyme. (C) 1996 Academic
Press, Inc.