C. Zijlstra et al., ARABIDOPSIS-THALIANA EXPRESSING THE CAULIFLOWER MOSAIC-VIRUS ORF-VI TRANSGENE HAS A LATE FLOWERING PHENOTYPE, Virus genes, 13(1), 1996, pp. 5-17
Expression of open reading frame (ORF) VI of cauliflower mosaic virus
(CaMV) in transgenic Arabidopsis thaliana caused a typical syndrome ch
aracterised by leaf chlorosis, vein clearing, plant stunting and reduc
ed fertility. In addition and in comparison to untransformed controls
we observed the formation of much larger rosettes of leaves combined w
ith much later flowering and more extensive tillering. In these aspect
s, the ORF VI transgenic plants resembled late flowering mutants, All
these phenotypes correlated with expression of ORF VI in three lines o
f transgenic plants which were produced independently, with different
Ti-plasmid derived vectors and with different selective markers. The l
ate flowering phenotype cosegregated with the transgene.