Aef. Nassar et al., ELECTROCHEMISTRY OF CAST FILMS OF FERREDOXIN AND LIPID BILAYERS ON ELECTRODES, Journal of electroanalytical chemistry [1992], 416(1-2), 1996, pp. 183-185
Structural stability and electrochemistry of chlorella ferredoxin immo
bilized in a cast film of molecular bilayer membranes of a biological
lipid, dimyristoylphosphatidylcholine (DMPC) on a quartz substrate or
a pyrolytic graphite electrode were examined, A specular reflectance s
pectrum of the film showed that the [2Fe-2S] cluster in ferredoxin ret
ains its original structure even in an air atmosphere. This indicates
that the lipid bilayers provide suitable microenvironments for a stabl
e immobilization of the protein. Cast films of ferredoxin+DMPC on a gr
aphite electrode gave well-defined, nearly reversible redox waves with
a formal potential of -0.59 V vs. Ag/AgCl, which is close to the repo
rted value at promoter-modified electrodes, indicating that the electr
ochemical communication is from native ferredoxin.