GENETIC SELECTION-STRATEGIES FOR GENERATING AND CHARACTERIZING CATALYSTS

Authors
KAST P HILVERT D
Citation
P. Kast et D. Hilvert, GENETIC SELECTION-STRATEGIES FOR GENERATING AND CHARACTERIZING CATALYSTS, Pure and applied chemistry, 68(11), 1996, pp. 2017-2024
Citations number
102
Categorie Soggetti
Chemistry
Journal title
Pure and applied chemistry → ACNP
ISSN journal
00334545
Volume
68
Issue
11
Year of publication
1996
Pages
2017 - 2024
Database
ISI
SICI code
0033-4545(1996)68:11<2017:GSFGAC>2.0.ZU;2-3
Abstract
Molecular evolution is a powerful tool for exploring and creating cata lytic function in biological macromolecules. This review focuses on ex amples and current strategies for the application of random mutagenesi s of target genes coupled with direct selection in vivo. The technolog y is illustrated by the dissection of catalytic features in chorismate mutase. Future avenues for identifying and evolving catalytic antibod ies are also discussed.