3'-NONTEMPLATED A-ADDITION BY TAQ DNA-POLYMERASE - AN ADVANTAGE IN THE CONSTRUCTION OF SINGLE AND DOUBLE MUTANTS

Authors
JAGATHREDDY J RAO NA SAVITHRI HS
Citation
J. Jagathreddy et al., 3'-NONTEMPLATED A-ADDITION BY TAQ DNA-POLYMERASE - AN ADVANTAGE IN THE CONSTRUCTION OF SINGLE AND DOUBLE MUTANTS, Current Science, 71(9), 1996, pp. 710-712
Citations number
12
Categorie Soggetti
Multidisciplinary Sciences
Journal title
Current Science → ACNP
ISSN journal
00113891
Volume
71
Issue
9
Year of publication
1996
Pages
710 - 712
Database
ISI
SICI code
0011-3891(1996)71:9<710:3ABTD->2.0.ZU;2-1
Abstract
The 3'-end non-templated 'A' addition by Tag DNA polymerase has been d escribed as a disadvantage in the generation of site-specific mutants as this 'A' leads to a unplanned second mutation, Here we demonstrate the utility of this 3' non-templated 'A' addition in the simultaneous construction of single and double mutants of serine hydroxymethyltrans ferase.