DIFFERENTIATION OF PASTEURELLA-MULTOCIDA SUBSPECIES MULTOCIDA ISOLATES FROM THE RESPIRATORY SYSTEM OF PIGS BY USING POLYMERASE CHAIN-REACTION FINGERPRINTING TECHNIQUE
B. Zucker et al., DIFFERENTIATION OF PASTEURELLA-MULTOCIDA SUBSPECIES MULTOCIDA ISOLATES FROM THE RESPIRATORY SYSTEM OF PIGS BY USING POLYMERASE CHAIN-REACTION FINGERPRINTING TECHNIQUE, Journal of veterinary medicine. Series B, 43(10), 1996, pp. 585-591
PCR fingerprinting technique was applied to subtype 44 Pasteurella mul
tocida subspecies multocida (P.m.sp.m.) isolates from the respiratory
system of pigs. Two single primers were tested for their abilities to
generate individual fingerprints by using PCR. Primer 1 (core sequence
of the M13 phage) grouped the 44 P.m.sp.m. strains into five distinct
fingerprinting profiles, while primer 2 ((GACA)(4)) grouped them into
seven profiles. The results suggest that PCR fingerprinting is an eff
icient technique to detect DNA polymorphism in the:species P.m.sp.m. T
his technique could be used to differentiate P.m.sp.m. strains of the
same capsular serotype.