STRUCTURES OF CELL-WALL POLYSACCHARIDES FROM SUSPENSION-CULTURED CELLS OF CRYPTOMERIA-JAPONICA

Cell walls isolated from suspension-cultured cells of Cryptomeria japo nica D. Don (sugi) were treated by sequential extraction with trans-1, 2-cyclohexanediamine-N,N,N',N'-t acid (CDTA), Na2CO3,1 M and 4 M KOH s olutions to isolate cell-wall polysaccharides. Most parts of the pecti n were solubilized with 0.05 M CDTA and Na2CO3 solutions, and total yi elds of these fractions amounted to about 20% of the cell walls. These fractions consisted of homogalacturonan and rhamnogalacturonan. Hemic ellulosic polysaccharides extracted with 1 M and 4 M KOH solutiuons we re comprised mainly of glucomannan and xyloglucan. The main component of the extract residue was cellulose. Pectin and hemicellulose still r emained in the residue. The cell-wall polysaccharides of suspension-cu ltured sugi cells consisted of 23.7% pectic polysaccharides, 17.9% xyl oglucan, and 10.2% glucomannan. The other components were traces of xy lan and cellulose. The polysaccharide composition of the suspension-cu ltured sugi was similar to that of the xylem differentiating zone of s ugi. Futhermore, except for the presence of glucomannan, suspension-cu ltured sugi (gymnosperm) had the same cell-wall polysaccharides as tho se of suspension-cultured sycamore maple (Acer pseudoplatanus L.; angi osperm) cells.