Sh. Brorson et F. Skjorten, THE THEORETICAL RATIO OF IMMUNOGOLD LABELING OF DEPLASTICIZED EPOXY SECTIONS AND ACRYLIC SECTIONS, Micron, 27(3-4), 1996, pp. 203-209
The purpose of this study was to predict the ratio of immunogold label
ing of deplasticized epoxy sections and LR-White sections on the basis
of theoretical considerations. Tissues used in the experiments were p
ancreas, pituitary, kidney, thyroid, and fibrin. Antigens used as test
proteins were glucagon, somatostatin, thyroglobulin, chromogranin A,
ACTH (= Adrenocorticotropt hormone), amyloid A, and fibrinogen. These
are proteins of different sizes. The quotient labeling(deplasticized)/
labeling(LR-white) was deduced theoretically and compared to measureme
nts based on immunogold experiments to obtain a theoretical model with
acceptable correlation to the measurements. This study describes a th
eory-expressed mathematically-for what happens at the molecular level
in immunoelectron microscopy at the surface of deplasticized epoxy sec
tions and acrylic sections. The theory explains why we normally get ab
out the same amount of immunogold labeling using LR-White sections (ac
rylic resin) and deplasticized epoxy sections. Taking the nuances into
account, the theory indicates increased usefulness of deplasticized e
poxy sections when the diameter of the protein carrying the epitopes d
ecreases. Copyright (C) 1996 Elsevier Science Ltd.