CD4(-CELLS FROM ADULTS RESPOND TO RECALL ANTIGENS AFTER CD28 LIGATION() CD45RA(+) T)

The leukocyte common antigen isoforms CD45RA and CD45RO have long been used to discriminate human naive and memory T cells respectively. Thi s model was largely based on the observation that CD45RO(+) T cells re spond preferentially to and show a higher frequency of precursors spec ific for recall antigens. However, CD45RA(+) T cells have more stringe nt requirements for stimulation and standard in vitro assays may favou r CD45RO(+) cells in this respect. We tested the hypothesis that CD45R A(+) T cells respond poorly to in vitro stimulation with recall antige ns because of inadequate stimulation rather than a lack of precursors. Limiting dilution analyses (LDA) for tetanus toroid (TT)-specific T c ells were performed in the presence or absence of erogenous anti-CD28 antibody. Addition of anti-CD28 yielded no proliferation in the absenc e of specific antigen. The precursor frequency for TT in the CD4(+) CD 45RO(+) population was similar to 1:4000, while the frequency of CD4() CD45RA(+) T cells specific for TT was 4- to >20-fold lower. Addition of anti-CD28 antibody did not significantly alter the apparent precur sor frequency for CD45RO(+) cells but yielded an enhancement of the va lue for CD45RA(+) cells by 3- to >5-fold. No enhancement of antigen-sp ecific proliferation by anti-CD28 was observed with CD45RA(+) T cells derived from cord blood, although phytohemagglutinin responses of thes e cells were amplified by CD28 antibody. These results indicate that c onventional LDA underestimate the true precursor frequency of antigen- specific cells within the adult CD45RA(+) population and support the p ossibility that a small number of cells revert from a primed (CD45RO()) to an unprimed (CD45RA(+)) state. The majority of memory T cells, h owever, appear to reside in the CD45RO(+) population