M. Kadowaki et al., PARTICIPATION OF 5-HT3, 5-HT4, AND NICOTINIC RECEPTORS IN THE PERISTALTIC REFLEX OF GUINEA-PIG DISTAL COLON, American journal of physiology: Gastrointestinal and liver physiology, 34(5), 1996, pp. 849-857
The roles of 5-hydroxytryptamine(3) (5-HT3), 5-HT4, and nicotinic rece
ptors in the peristaltic reflex were investigated in isolated segments
of guinea pig distal colon. The reflex, assessed by measuring the pro
pulsion of solid pellets, was affected neither by 5-HT3-selective anta
gonists (ondansetron, granisetron) nor by 5-HT4-selective antagonists
(SDZ-205-557, GR-113808A, SE-204070) applied individually (1.0 mu M);
nevertheless, the reflex was inhibited by combining these antagonists
or by applying a 5-HT3/5-HT4 dual antagonist (FK-1052). Hexamethonium
abolished the peristaltic reflex at 100 mu M, but not at 10-32 mu M. I
n contrast, the peristaltic reflex was inhibited when hexamethonium (3
2 mu M) was combined with either a 5-HT3- or 5-HT4-selective antagonis
t (1.0 mu M). These observations suggest that 5-HT3, 5-HT4, and nicoti
nic receptors participate in the initiation and/or propagation of the
peristaltic reflex. The data are consistent with the idea that these r
eceptors are arranged in parallel in the neural pathways that mediate
the peristaltic reflex in the distal colon.