MICROMANIPULATION IMPROVES IN-VITRO FERTILIZATION RESULTS AFTER EPIDIDYMAL OR TESTICULAR SPERM ASPIRATION IN PATIENTS WITH CONGENITAL ABSENCE OF THE VAS-DEFERENS

In all, 58 couples suffering from infertility because of congenital bi lateral absence of the vas deferens underwent a total of 67 combined m icrosurgical epididymal aspiration or testicular sperm extraction (TES E) and in-vitro fertilization (IVF) treatments, The oocytes recovered were inseminated by either the microdroplet IVF technique (n = 20), su bzonal insemination (SUZI; n = 10) or intracytoplasmic sperm injection (ICSI; n = 37), Of the ICSI cycles, 12 were performed using spermatoz oa obtained by TESE, Fertilization rates for epididymal spermatozoa we re significantly higher for SUZI (17.9%, 17/95) and ICSI (34.4%, 137/3 98) than for microdroplet IVF (5.2%, 18/343) cycles, The proportion of cycles in which fertilization was achieved was higher in the SUZI (80 %) and ICSI (95%) cycles than in the IVF cycles (45%), Delivery or an ongoing pregnancy was achieved in one (5%) IVF cycle, two (20%) SUZI c ycles and seven (18.9%) ICSI cycles, SUZI or ICSI using epididymal or testicular spermatozoa significantly improved the oocyte fertility rat e, The ICSI procedure was especially advantageous in patients for whom spermatozoa were obtained from a testicular biopsy.