CHANGES IN NUCLEAR MORPHOLOGY IN THE HUMAN ENDOMETRIAL GLANDULAR EPITHELIUM IN WOMEN WITH UNEXPLAINED INFERTILITY

In the light and electron microscopical study reported here, we docume nt the structure of the nucleus, nucleolus and nuclear channel system (NCS) in the uterine glandular epithelium in both fertile and infertil e women during the early luteal phase, Nuclear volume was found to be larger in the infertile group at day 5 after the luteinizing hormone s urge (LH+5) compared to day-matched fertile subjects, A two-way analys is of variance performed on nucleolar volume data from fertile and inf ertile women biopsied on days LH+4, +5 and +6 revealed a significant e ffect of condition but no effect of day or interaction. Nucleolar volu me decreased from day LH+2 to day LH+6 in the fertile group, the sharp est decrease occurring between day LH+3 and day LH+4, The largest mean volume of the NCS was found at day LH+5 in the fertile group and day LH+6 in the infertile group. The results suggest a delay in the develo pment of this organelle in the infertile women. The present study has documented alterations in the nuclei of uterine glandular cells from i nfertile patients, In these infertile women, there is also a delayed e laboration of the secretory apparatus and this delay correlates well w ith the delayed/reduced expression of a luteal-specific glycoprotein.