V. Pancre et al., INDUCTION OF CYTOTOXIC T-CELL ACTIVITY BY THE PROTECTIVE ANTIGEN OF SCHISTOSOMA-MANSONI SM28GST OR ITS DERIVED C-TERMINAL LIPOPEPTIDE, Scandinavian journal of immunology, 44(5), 1996, pp. 485-492
In a previous work the authors demonstrated that immunization with Sch
istosoma mansoni 28-kDa glutathion-S-transferase (Sm28GST) was able to
reduce hepatic damage in infected mice and that the adoptive transfer
of Sm28GST-specific T cells reproduced the protective effect obtained
with the recombinant molecule. In the present paper, the authors show
that Sm28GST is also able to stimulate an antigen-specific, cytotoxic
T-cell response against Sm28GST-pulsed P815 target cells in normal mi
ce and that effector cells induced in vivo were classical Class I MHC-
restricted CD8(+) lymphocytes, The authors found no spontaneous CTL ac
tivity against Sm28GST-pulsed target cells during the course of the in
fection by S. mansoni although Sm28GST is expressed at different devel
opmental stages of the parasite. It was observed, however, that immuni
zation with Sm28GST is sufficient to elicit a significant level of CTL
response for 6 weeks in infected mice. The role of these Class I MHC-
restricted CD8(+) lymphocytes in the protection observed precisely at
the same period in immunized mice remains to be elucidated. The author
s also observe that immunization with the lipopeptidic form of the C-t
erminal peptide of the molecule (190-211 peptide) led to a CTL activat
ion comparable to that observed after immunization with the whole mole
cule demonstrating the feasibility of using a synthetic lipopeptide as
immunogen for a CTL response against Sm28GST epitopes. Moreover, like
Sm28GST-specific CTLs, 190-211 lipopeptide-specific cells were also C
lass I MHC-restricted lymphocytes.