CLONING AND EXPRESSION OF THE CDNA FOR MOUSE NAD(-DEPENDENT 15-HYDROXYPROSTAGLANDIN DEHYDROGENASE())

The cDNA for mouse NAD(+) dependent 15-hydroxyprostaglandin dehydrogen ase (15-PGDH) was isolated from a lung cDNA library. The cDNA contains a 798 bp open reading frame that codes for a protein of 266 amino aci ds (M(r) 28 775) which shares 87% identity with the human 15-PGDH prot ein. The regions that an believed to form the NAD(+) binding site and the active site are conserved in the mouse and human enzymes. The auth enticity of the mouse cDNA was confirmed by expression of an active 15 -PGDH in Escherichia coli. Northern blot analysis demonstrated that 15 -PGDH mRNA is expressed primarily in lung, intestine, stomach and live r.