ISOLATION AND CHARACTERIZATION OF THE BACILLUS-SUBTILIS TRYPTOPHANYL-TRANSFER-RNA SYNTHETASE GENE (TRPS) CONFERRING 5-FLUOROTRYPTOPHAN RESISTANCE AND TEMPERATURE SENSITIVITY

Authors
CHOW KC WONG JTF
Citation
Kc. Chow et Jtf. Wong, ISOLATION AND CHARACTERIZATION OF THE BACILLUS-SUBTILIS TRYPTOPHANYL-TRANSFER-RNA SYNTHETASE GENE (TRPS) CONFERRING 5-FLUOROTRYPTOPHAN RESISTANCE AND TEMPERATURE SENSITIVITY, Biochimica et biophysica acta, N. Gene structure and expression, 1309(1-2), 1996, pp. 42-46
Citations number
9
Categorie Soggetti
Biology,Biophysics,"Biothechnology & Applied Migrobiology
Journal title
Biochimica et biophysica acta, N. Gene structure and expression → ACNP
ISSN journal
01674781
Volume
1309
Issue
1-2
Year of publication
1996
Pages
42 - 46
Database
ISI
SICI code
0167-4781(1996)1309:1-2<42:IACOTB>2.0.ZU;2-#
Abstract
The mutant trpS gene of Bacillus subtilis encoding a thermosensitive t ryptophanyl-tRNA synthetase that also confers resistance to growth inh ibition by 5-fluorotryptophan at permissive temperature has been isola ted and characterized. A point mutation at codon 82 of the gene from a wild-type TCA codon for Ser to a TTA codon for Leu has been identifie d.