ACTIN MESSENGER-RNA IS NOT LOWERED IN CHRONIC MYELOID LEUKEMIC GRANULOCYTES

Chronic myeloid leukemic (CML) granulocytes exhibit defects in several functions, some of which have been associated with changes in the exp ression of cell surface molecules, actin reorganization and lowered le vels of total cellular actin. In this study, we show by northern blott ing that the steady-state level of mRNA for actin is not decreased in the CML granulocyte. Our data suggest that the lowered levels of actin protein in the leukemic granulocyte may be due to altered control at the translational/post-translational step, rather than at the level of transcription/post-transcription, implicated in the regulation of exp ression of the surface molecules, Fc gamma RII, Fc gamma RIII and alka line phosphatase. Copyright (C) 1996 Elsevier Science Ltd.