As. Shashkov et al., STRUCTURE OF THE O-SPECIFIC POLYSACCHARIDE OF THE BACTERIUM PROTEUS-MIRABILIS O-10 CONTAINING L-ALTRURONIC ACID, A NEW COMPONENT OF O-ANTIGENS, Biochemistry, 61(9), 1996, pp. 1100-1105
An acidic O-specific polysaccharide was obtained by mild acid degradat
ion of the lipopolysaccharide of the bacterium Proteus mirabilis O10 a
nd studied by full acid hydrolysis, carboxyl reduction, and H-1- and C
-13-NMR spectroscopy, including two-dimensional correlation spectrosco
py (COSY4), H-detected heteronuclear H-1, C-13 multi-quantum coherence
(HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (RO
ESY). It was found that the polysaccharide contains 2-acetamido-2-deox
y-D-glucose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid, and
L-altruronic acid, and the following structure of the branched tetras
accharide repealing unit of the polysaccharide was established: [GRAPH
ICS] t azide-facilitated decay of the M intermediate and shift the lin
ear part of the dose-response curve of the effect of the azide concent
ration on the intermediate M decay rate to higher azide concentrations
. The efficiency of azide as an accelerator of the M intermediate deca
y in these two populations decreases 11- and 140-fold in the presence
of glutaraldehyde and 200- and 1600-fold in the presence of LuCl3. Gly
cerol and sucrose induce deceleration of the M intermediate decay in t
he wild-type bacteriorhodopsin and in the D96N mutant in the presence
of azide, A scheme for the photocycle is suggested that assumes an equ
ilibrium of two M intermediate forms differing in permeability for azi
de and, probably, for water molecules.